DNA and RNA CleanUp

AVAILABLE KIT FORMATS

Catalog No#	Description	Number of Reactions*
CNGS-0001	CleanNGS (1 mL)	55
CNGS-0050	CleanNGS (50 mL)	2,777
CNGS-0500	CleanNGS (500 mL)	27,777

*Based upon a 10 µl PCR reaction volume

CleanNGS

DNA and RNA clean-up for next generation sequencing library construction

Since its introduction in 2005, Next Generation Sequencing (NGS) opened many doors in the fields of translational genomics and molecular diagnostics by massive parallel decoding of DNA or RNA fragments. To generate high quality NGS data, preparation of pure DNA or RNA of a specific length is one of the key process steps. We offer our CleanNGS for library cleanup and size selection to make this process simple and reliable.

Our special buffer formula ensures optimal size selection for NGS libaries and the high quality magnetic beads allow faster separations and better RNA/DNA recovery. CleanNGS is produced RNase free, which makes it an ideal solution for all downstream RNA or DNA NGS experiments.

CleanNGS is suited for cleanup in between library prepping steps, after library prepping and for size selection in next-generation sequencing or sanger sequencing. It is also a highly efficient cleanup method to use before other techniques such as PCR, cloning or CRISPR-Cas9 to improve the quality of your results. Our purification reagent provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).

Based on CleanNA’s proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.

Workflow

For double sided size selection, we first add CleanNGS reagent with magnetic beads in a certain volume ratio. Separate the large DNA or RNA fragments from the solution with a magnetic plate and add more CleanNGS reagent to the supernatant to clean up the small DNA fragments and inhibitors. After two washing steps, the purified DNA or RNA is eluted.

Amplicons purified with CleanNGS system are ready to be used in the following applications:

Next-Generation Sequencing
PCR
Genomic DNA clean up
RNA clean up
Fragment Analysis
Microarrays
Restriction enzyme clean up
Cloning

Benefits

Reviews

Good quality and value for money

We have been using cleanNGS for several years now at ILVO for clean-up of Illumina targeted sequencing libraries and clean-up of Nanopore WGS libraries. It replaces the AMPureXP beads 1:1 for several workflows and we are very happy with the consistently high quality, good value for money and simple protocol.

Application Area: NGS clean-up

Review Date: 21 Oct 2021 | CleanNA

Steve Baeyen , ILVO

Average Rating 5.0

Easy to use, time efficient, lower cost for more samples.

Purification of DNA with clean NGS is time efficiënt compared to the use of purification columns. The protocol is straightforward and easy to follow. You can purify more samples at once, so it is handy for high throughput. The total cost gets lower the more samples you process.

Application Area: Purification PCR material for Miseq

Review Date: 26 Jul 2021 | CleanNA

Isabel Vanoverberghe, Kulak

Average Rating 4.0

Very reliable products that I confidently entrust my precious samples with.

At our lab the magnetic bead based plant DNA purification system Clean Plant PK DNA Kit is routinely used for quick and robust gDNA isolation from various plant species. The ease of use and compatibility to our liquid handler made it one of our most preferred plant DNA extraction protocols. After implementing the CleanNGS magnetic beads in our lab routines we covered most of our plant DNA handling and purification methodologies. The CleanNGS magnetic beads substitute many of other manufacturers magnetic beads with comparable or even better results when applied for size selection and purification of our plant DNA extracts. Clean NGS products helped our lab to improve quality and throughput and substantially cut costs and hands-on-time.

Application Area: Extraction and cleanup of plant DNA

Review Date: 17 Jul 2020 | CleanNA

Almer van Belzen, Genetwister Technologies

Average Rating 5.0

Very happy and will keep using them.

Some members of the lab were not keen to change away from the regular bead clean up supplier. We tested these and found they gave slightly higher amount of DNA and just as clean.

Application Area:Clean up libraries for next generation sequencing

Review Date:29 Jun 2020 | CleanNA

Chris Kimberley, Barts Cancer Institite

Average Rating 5.0

Easy to use!

This product was used as part of my research project in molecular epidemiology and I was really happy with the results it produced. It made it an easy process to extract the DNA across all samples and ensured accurate sequencing data.

Application Area: DNA extraction

Review Date: 8 Jun 2020 | CleanNA

Jonathan Abeles, London School of Hygiene and Tropical Medicine

Average Rating 4.3

Like-for-like replacement to the large name-brand equivalents.

Delivers reproducible DNA size-selection for a variety of NGS applications and can be used in with-bead applications.

Application Area: NGS library prep

Review Date: 16 Jan 2020 | CleanNA

Patrick van Eijk, Division of Cancer & Genetics, School of Medicine, Cardiff University

Average Rating 4.7