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Cell-free DNA (cfDNA) has experienced a remarkable rise in interest as a diagnostic and research tool in medicine. Over the last years, cfDNA had a major impact on prenatal diagnostics by enabling non-invasive prenatal testing (NIPT). Furthermore, it is rapidly evolving into the new diagnostic standard for oncology, transplant medicine, and cardiovascular diseases. Our CE-IVD marked Clean Cell Free DNA Kit helps laboratories with the first step of the diagnostic process: extracting the cfDNA from human plasma.
Our kit extracts cfDNA fragments from 1 mL human plasma in a three-step procedure that is easy to automate. The unique lysis buffer releases the DNA from proteins and vesicles and the optimised buffer combination enriches DNA fragments ranging from 120 to 400 base pairs. Combined with the CE-IVD marking, this makes the Clean Cell Free DNA Kit the ideal start to reliable and reproducible qPCR results in molecular diagnostics.
• Prenatal testing (NIPT)
• Oncology
• Transplant medicine
• Cardiovascular diseases
• Post-trauma monitoring
Initially, the uniquely formulated lysis buffer is added to the plasma sample to release the cfDNA from proteins and vesicles, while DNases and RNases are inactivated. After binding of the cfDNA to the added magnetic beads, the cfDNA-magnetic bead complex can be separated from the lysate by using a magnetic separation device. A series of quick wash steps remove trace contaminants and the purified cfDNA can be eluted from the magnetic beads using the Elution Buffer.
Enrichment of short DNA fragments
FIGURE 1.
Electropherogram of 8 different human plasma samples after extraction with the Clean Cell Free DNA Kit.
| qPCR analysis
FIGURE 2. Albumin directed TaqMan® qPCR performed with Sensifast Lo-Rox after extracting DNA from 13 human plasma with the Clean Cell Free DNA Kit and Company Q. |
High DNA yield
FIGURE 3. Yield after extracting DNA from 13 human plasma samples with the Clean Cell Free DNA Kit and Company Q, measured with the DeNovix DS-11 FX. |
| Catalog No# | Size |
| CCF-D0384 | 384 preps |
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CleanNA
Coenecoop 75
2741 PH Waddinxveen
The Netherlands
V.A.T. – NL859255955B01
KVK – 72839511
T: +31 (0) 182 22 33 50
E: info@cleanna.com
Technical support
E: support@cleanna.com
