CleanPCR

High recovery PCR clean up system

CleanNA will tell you more about our CleanPCR and it’s relation to sanger sequencing. The CleanPCR kit is an efficient PCR and Next Gen library prep clean up system based on paramagnetic beads technology, providing an efficient purification of PCR amplicons. With its simple, three-step protocol, CleanPCR removes salts, primers, primer-dimers, dNTPs, while DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Benefits

High recovery of amplicons greater than 100 bp
Efficiently removes unincorporated dNTPs, primers, primer dimers and other contaminants
Stable and high recovery of PCR products post-cleanup
No centrifugation or filtration

Applications

Amplicons purified with CleanPCR system are ready to be used in the following applications:

• PCR
• Mutation detection and Genotyping
• Sequencing (Sanger and Next Generation)
• Fragment Analysis
• Microarrays
• Restriction enzyme clean up
• Cloning

We would also like to recommend the Clean Viral DNA/RNA kit, used for DNA and RNA isolation!

Catalog No# Volume Number of reactions – 96 well format*
CPCR-0001 1 mL 27
CPCR-0050 50 mL 1388
CPCR-0500 500 mL 13888

*Based upon a 20 µl PCR reaction volume

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